Bioinformatics Study of The Expressed Sequence Tags of Salt Tolerance Genes from Mangrove Plant Rhizophora stylosa

2nd International Conference on Biosciences

Basyuni, M.^1 ; Baba, S.^2 ; Wati, R.^1 ; Oku, H.^3

Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1 Medan, North-Sumatera

20155, Indonesia^1

International Society for Mangrove Ecosystems, Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa

903-0213, Japan^2

Molecular Biotechnology Group, Tropical Biosphere Research Center, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa

903-0213, Japan^3

关键词: Bioinformatics analysis;    Chloroplast;    Expressed sequence tags;    Phylogenetic trees;    R. stylosa;    Salt tolerance;    Subcellular localizations;    Triterpenoids;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/197/1/012039/pdf
DOI  :  10.1088/1755-1315/197/1/012039

来源: IOP

【 摘 要 】

The present study describes the bioinformatics analysis of 13 expressed sequence tags (ESTs) of salt tolerance genes in mangrove plant, Rhizophora stylosa as well as homology, phylogenetic, sequence analysis, potential peptide, and subcellular localization. The DNA sequence among the ESTs from R. stylosa exhibited 50-97% homology between themselves. The target peptide value of chloroplast and mitochondrial varied from 0.071-0.800, 0.053 to 0.254, respectively, indicated it was possible to exist. Sub-cellular of the fragment genes mostly was in the plasma membrane and endoplasmatic reticulum. On the other hand, a few genes restored in golgi bodies, vacuole, and lysosome. These results suggested the importance of understanding the function of properties of the probably salt tolerance genes in R. stylosa genomic library. To clarify the relationship among the ESTs in R. stylosa, a phylogenetic tree was constructed. The phylogenetic tree depicts that there are three branches, the first branch contained one EST, the second cluster consists of 9 genes, in which the majority ESTs resides and the last group comprised of 3 ESTs. The present study, therefore, suggested the diversity of salt tolerance genes form discrete clusters in the phylogenetic tree.