Gliotoxicity, reverse transcriptase activity and retroviral RNA in monocyte/macrophage culture supernatants from patients with multiple sclerosis

FEBS Letters

Geny, Christian⁷  Amouri, Rim³  Ménard, Armelle³  Belliveau, James⁵  Malcus-Vocanson, Carine⁴  Mandrand, Bernard⁴  Rieger, François³  Lyon-Caen, Olivier⁸  Perron, Hervé²  Michel, Marlène²  Brouillet, Arthur³  Marcel, Frédérique²  Dobránsky, Tomás³  Deforges, Lionel¹  Armstrong, Marilyn⁶ 

[1]Service de Bactériologie-Virologie, Hôpital Henri Mondor, Créteil, France

[2]Laboratoire de Virologie moléculaire médicale, Université Joseph Fourier, La Tronche, France

[3]INSERM, Laboratoire de Neuromodulations Interactives et Neuropathologies, Paris, France

[4]Unité mixte CNRS-BioMérieux UMR 103, Lyon, France

[5]Department of Chemistry, Providence College, Providence, RI 02918, USA

[6]Department of Microbiology and Immunology, The Queen's University of Belfast, Belfast, UK

[7]Service de Neurologie, Hôpital Henri Mondor, Créteil, France

[8]Fédération de Neurologie and INSERM U.360, Hôpital de la Salpêtrière, Paris, France

关键词: Multiple sclerosis;    Monocyte;    Astrocyte;    Oligodendrocyte;    Cytotoxicity;    Retrovirus;    MS;    multiple sclerosis;    MMC;    monocyte/macrophage culture(s);    MMCS;    monocyte/macrophage culture supernatant(s);    MSRV;    multiple sclerosis associated retrovirus;    ERV;    human endogenous retrovirus;    PLL;    poly l-lysine;    MTT;    3-(4;    5-dimethylthiazol-2-yl)-2;    5-diphenyl tetrazolium bromide;    RT;    reverse transcriptase;    GFAP;    glial fibrillary acidic protein;    PLP;    myelin proteolipid protein;    GC;    galactocerebroside;    MBP;    myelin basic protein;    ELOSA;    enzyme-linked oligosorbent assay;   

DOI  :  10.1016/S0014-5793(97)00889-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In investigating a possible link between a novel retroviral agent (provisionally called MSRV), recently characterised in multiple sclerosis (MS), and the neuropathology of MS, it was found that there was a significant correlation between gliotoxicity and reverse transcriptase activity in monocyte/macrophage culture supernatants (MMCS) unique to MS patients. MMCS from healthy controls and patients with other neurological diseases did not display either gliotoxicity or reverse transcriptase activity. The observed gliotoxic effect was an initial, intermediate filament network disorganization and subsequent cell death which was specific to astrocytes and oligodendrocytes. The reverse transcriptase activity and MSRV-specific RNA were observed during the first 2 weeks of culture in MMCS from patients with active MS. The further elucidation of the molecular form(s) of this gliotoxic factor and its original source may be crucial in elucidating important etiopathogenic mechanisms in MS.

【 授权许可】

Unknown