APP RNA splicing is not affected by differentiation of neurons and glia in culture

FEBS Letters

Lübbert, Hermann²  Dutly, Fabrizio²  Frei, Karl¹  Foguet, Montserrat²  Paul, Gisela² 

[1] University Hospital Zürich, Häldeliweg 4, CH-8044 Zürich, Switzerland;Preclinical Research, Sandoz Pharma Ltd., CH-4002 Basel, Switzerland

关键词: APP gene expression;    Neuron;    Astrocyte;    Microglial cell;    Oligodendrocyte;   

DOI  :  10.1016/0014-5793(92)80706-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Amyloid precursor protein (APP) gene expression was investigated in primary cultures of neurons, astrocytes, microglial cells and oligodendrocytes. Neurons from various rat brain regions, as well as oligodendrocytes, contained RNA encoding APP₆₉₅, while astrocytes and microglial cells expressed high levels of RNAs for APP₇₇₀ and APP₇₅₁. It was studied whether the cell type-specific regulation of APP gene expression could be modified by induction of cellular differentiation in vitro. While neuronal differentiation of PC12 cells has been shown to correspond with an altered pattern of APP splicing, in the primary cultures neither the time in culture nor a treatment of the cells with appropriate differentiation factors affected this pattern.

【 授权许可】

Unknown