Possible involvement of radical reactions in desialylation of LDL

FEBS Letters

Tokumaru, Sadako²  Kojo, Shosuke¹  Tanaka, Kyoko¹ 

[1]Department of Food Science and Nutrition, Nara Women's University, Nara 630, Japan

[2]Department of Life and Health Sciences, Joetsu University of Education, Joetsu, Niigata 943, Japan

关键词: Low density lipoprotein;    LDL;    Sialic acid;    Desialylation;    Lipid peroxidation;    BHT;    butylated hydroxytoluene;    DMB;    1;    2-diamino-4;    5-methylenedioxy-benzene dihydrochloride;    HPLC;    high-performance liquid chromatography;    LDL;    low density lipoprotein;    NANA;    N-acetylneuraminic acid (sialic acid);   

DOI  :  10.1016/S0014-5793(97)00917-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of oxidatively modified LDL in the pathogenesis of atherosclerosis has been well documented. These studies have focused on modifications of lipid and protein parts of LDL. Recently desialylated LDL has received attention in relation to atherosclerosis and coronary artery disease. We examined the possible involvement of radical reactions in desialylation of LDL. Human LDL was subjected to oxidative damage using Cu²⁺ ion. As the conjugated dienes monitored by absorption at 234 nm increased, the content of sialic acid decreased steadily. Both the elevation of conjugated diene and the decrease of sialic acid were inhibited by β-mercaptoethanol, a typical radical scavenger. Besides, both butylated hydroxytoluene and a nitrogen atmosphere inhibited the decrease of sialic acid. These inhibition experiments suggested that sialic acid moieties in LDL were reactive toward radicals.

【 授权许可】

Unknown