Ascorbic acid can either increase or decrease low density lipoprotein modification

FEBS Letters

Leake, David S.¹  Stait, Suzanne E.¹ 

[1] Department of Biochemistry and Physiology, School of Animal and Microbial Sciences, University of Reading, Whiteknights, PO Box 228, Reading,Berks, RG6 2AJ, UK

关键词: Antioxidant;    Ascorbic acid;    Atherosclerosis;    Low density lipoprotein;    Macrophage;    Vitamin C;    BHT;    butylated hydroxytoluene;    DMEM;    Dulbecco's modified Eagle's medium;    FCS;    foetal calf serum;    LDL;    low density lipoprotein;    MDA;    malondialdehyde;    TBARS;    thiobarbituric acid reactive substances;   

DOI  :  10.1016/0014-5793(94)80469-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In freshly prepared low density lipoprotein (LDL), ascorbate inhibited LDL oxidation by macrophages at the higher concentrations tested (60–100 μM). In contrast, with LDL that had been allowed to autoxidise in the refrigerator (3°C) for at least 10 weeks after isolation (mildly oxidised or minimally-modified LDL), ascorbate did not inhibit the modification of LDL in the presence of macrophages. Ascorbate actually modified autoxidised LDL itself in the absence of macrophages to greatly increase its uptake by macrophages. The modification of autoxidised LDL by ascorbate increased the levels of thiobarbituric acid-reactive substances in the medium and was completely inhibited by the antioxidant butylated hydroxytoluene. Thus the effects of ascorbate on unoxidized LDL can be very different to those on mildly oxidised LDL.

【 授权许可】

Unknown