Formation of N‐formylkynurenine suggests the involvement of apolipoprotein B‐100 centered tryptophan radicals in the initiation of LDL lipid peroxidation

FEBS Letters

Esterbauer, Hermann¹  Gieβauf, Andreas¹  van Wickern, Barbara²  Steinhart, Hans²  Simat, Thomas² 

[1]Institute of Biochemistry, University of Graz, Schubertstr. 1, A-8010 Graz, Austria

[2]Institute of Biochemistry and Food Chemistry, University of Hamburg, Grindelallee 117, D-20146 Hamburg, Germany

关键词: Low density lipoprotein;    Tryptophan;    N-Formylkynurenine;    Kynurenine;    Tryptamine;    Free radicals;    apo B;    apolipoprotein B;    LDL;    low density lipoprotein;    MeCN;    acetonitrile;    McOH;    methanol;    NFK;    N-formylkynurenine;    PBS;    phosphate buffered saline;    TFA;    trifluoroacetic acid;    0.1% in water;    Trp;    l-tryptophan;   

DOI  :  10.1016/0014-5793(96)00546-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tryptophan oxidation products were determined in pronase E digests of apo B of delipidated, native and Cu²⁺ oxidized LDL using a sensitive and specific HPLC method. Oxidized LDL contained N-formylkynurenine, kynurenine and tryptamine but no oxindolylalanine and 5-hydroxytryptophan. N-Formylkynurenine increased from an initial value of 0.21 to 1.67 mollmol apo B within 5 h. Apo B of native LDL also contained kynurenine (0.80 mollmol) and tryptamine (0.13 mol/mol). The results support the assumption that oxidation of Trp residues in apo B is an early event and possibly an elementary reaction involved in initiating LDL oxidation.

【 授权许可】

Unknown