Modified low density lipoproteins activate human macrophages to secrete immunoreactive endothelin

FEBS Letters

Martin-Nizard, Françoise¹  Houssaini, Hayat Sqalli¹  Fruchart, Jean-Charles¹  Lestavel-Delattre, Sophie¹  Duriez, Patrick¹ 

[1]Department of Lipoproteins and Atherosclerosis Research and U325 INSERM, Pasteur Institute, 1 rue du Professeur Calmette, BP 245, 59019 Lille Cédex, France

关键词: Endothelin;    Macrophage;    Low density lipoprotein;    ET;    endothelin;    TNF;    tumor necrosis factor;    PMA;    phorbol 12-myristate 13-acetate;    LDL;    low-density lipoprotein;    Ac-LDL;    acetylated LDL;    Ox-LDL;    oxidized LDL;    BHT;    butylated hydroxytoluene;    n-LDL;    native-LDL;    BHT-LDL;    butylated hydroxytoluene-LDL;    BHT-Ac-LDL;    butylated hydroxytoluene-Ac-LDL;    RIA;    radioimmunoassay;    PiA;    polyinosinic acid;    FCS;    fetal calf serum;    LPDS;    lipoprotein deficient serum;    MEM;    minimal essential serum;    PKC;    Protein kinase c;   

DOI  :  10.1016/0014-5793(91)81167-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study attempted to determine if low density lipoproteins (LDL) induce the production of endothelins (ET) by human macrophages. Non-protected LDL from macrophage induced oxidation (n-LDL), copper-oxidized LDL (Ox-LDL), acetylated-LDL (Ac-LDL), butylated hydroxytoluene-LDL (BHT-LDL), BHT-Ac-LDL, polyinosinic acid (PiA, 1.5μg/ml), phorbol myristate acetate (PMA; 0.5 μM) and BHT alone (20 μM) were studied. The different compounds had the following potency to stimulate the ET secretion: PMA>Ox-LDL>Ac-LDL>n-LDL>BHT-LDL>PiA>PiA+Ac-LDL>BHT. In conclusion, mod stimulated ET secretion by human macrophages.

【 授权许可】

Unknown