FEBS Letters | |
Endothelin‐induced intracellular Ca2+ mobilization through its specific receptors in murine peritoneal macrophages | |
Kato, Toshiyuki1  Hanasaki, Kohji1  Arita, Hitoshi1  Kishino, Junji1  | |
[1] Shionogi Research Laboratories, Shionogi and Co. Ltd., Fukushima-ku, Osaka 553, Japan | |
关键词: Endothelin; Receptor binding; Cytosolic free Ca2+; Peritoneal macrophages; Mouse; ET; endothelin; [Ca2+]i; intracellular free Ca2+ concentration; | |
DOI : 10.1016/0014-5793(91)80214-N | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We studied the presence of specific binding sites for endothelin (ET) and the effect of ET on cytosolic free Ca2+ concentration ([Ca2+]i) in murine thiogylcolate-activated peritoneal macrophages. Scatchard analysis for binding experiments using [125I]ET-1 or [125I]ET-3 revealed the existence of a single class of binding sites. The binding parameters (K??? and B???) for [125I]ET-1 were almost identical to those for [125I]ET-3. In addition, unlabeled 3 ET isopeptides (ET-1, ET-2 and ET-3) inhibited the specific binding of both ET-1 and ET-3 with similar inhibitory potencies. All 3 ET isopeptides caused an increase in [Ca2+]i in the same dose-dependent manner (0.01–100nM). These results demonstrate the existence of an ET receptor with the same affinity for all isoforms that mediates the ET-induced intracellular Ca2+ mobilization in murine peritoneal macrophages.
【 授权许可】
Unknown
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