Glyceraldehyde, but not cyclic AMP‐stimulated insulin release is preceded by a rise in cytosolic free Ca2+

FEBS Letters

Pozzan, Tullio¹  Ullrich, Susanne²  Wollheim, Claes B.² 

[1] Istituto di Patologia Generale, Via Loredan 16, 35100 Padova, Italy;Institut de Biochimie Clinique, University of Geneva, Sentier de la Roseraie, 1211 Geneva 4, Switzerland

关键词: Cyclic AMP;    Forskolin;    Cytosolic free Ca2+;    Insulin release;    Carbohydrate;    Membrane potential;   

DOI  :  10.1016/0014-5793(84)80972-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The dynamics of changes in membrane potential, cytosolic free Ca²⁺ [Ca²⁺]_i and immunoreactive insulin release were assessed in RINm5F cells. Membrane depolarization and a rise in [Ca²⁺]_i preceded the stimulation of insulin release by D-glyceraldehyde. Forskolin, which raised the cellular cyclic AMP levels, stimulated insulin release without changing membrane potential or [Ca²⁺]_i. It is concluded that cyclic AMP acts on insulin release not by mobilizing Ca²⁺ but by another, as yet undefined, mechanism.

【 授权许可】

Unknown