Cyclic AMP raises cytosolic Ca2+ and promotes Ca2+ influx in a clonal pancreatic β‐cell line (HIT T‐15)

FEBS Letters

Prentki, Marc¹  Geschwind, Jean-François¹  Glennon, Major C.¹  Corkey, Barbara E.¹  Matschinsky, Franz M.¹ 

[1] Department of Biochemistry and Biophysics and Diabetes Research Center, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA

关键词: cyclic AMP;    Forskolin;    D-Glucose;    cytosolic free Ca2+;    Ca2+ channel blocker;    Insulin release;   

DOI  :  10.1016/0014-5793(87)80884-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect on cytosolic Ca²⁺ concentration ([Ca²⁺]_i) of cAMP analogues and the adenylate cyclase-stimulating agents forskolin, isoproterenol and glucagon has been examined in an insulin-secreting β-cell line (HIT T-15) using fura 2. All these manipulations of the cAMP messenger system promoted a rise in [Ca²⁺]_i which was blocked by the Ca²⁺ channel antagonists verapamil and nifedipine or by removal of extracellular Ca²⁺. The action of the adenylate cyclase activator forskolin was glucose-dependent. The results suggest that cAMP elevates [Ca²⁺]_i in HIT cells by promoting Ca²⁺ entry through voltage-sensitive Ca²⁺ channels, not through mobilization of stored Ca²⁺. Activation of Ca²⁺ influx may be an important component of the mechanisms by which cAMP potentiates fuel-induced insulin release.

【 授权许可】

Unknown