Apoptosis induction by inhibitors of Ser/Thr phosphatases 1 and 2A is associated with transglutaminase activation in two different human epithelial tumour lines

FEBS Letters

von Zezschwitz, Caroline¹  Vorwerk, Hilke¹  Tergau, Frithjof¹  Steinfelder, Hans Jürgen¹ 

[1] Institute of Pharmacology and Toxicology, University of Göttingen, Robert-Koch-Strasse 40, D-37075 Göttingen, Germany

关键词: Ser/Thr phosphatase inhibitor;    Okadaic acid;    Calyculin A;    Apoptosis;    Transglutaminase;    HeLa cell;    KB cell;    MCF-7 cell;   

DOI  :  10.1016/S0014-5793(97)00896-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two epithelial tumour lines, HeLa and KB, were treated with okadaic acid and calyculin A, specific inhibitors of Ser/Thr phosphatases (PP), esp. PP1 and PP2A. Morphological criteria, analysis of DNA fragmentation and studies of membrane integrity revealed that both agents concentration- and time-dependently induced apoptosis at nanomolar concentrations which in these cells was associated with the stimulation of a transglutaminase activity. Since a non-functional derivative of okadaic acid did not affect cell viability apoptosis was apparently related to the inhibition of PP1 and PP2A. Membrane damage marker activity was delayed by at least 24 h when compared to nuclear alterations.

【 授权许可】

Unknown