Expression and characterization of PP7, a novel plant protein Ser/Thr phosphatase distantly related to RdgC/PPEF and PP5

FEBS Letters

Evans, David E¹  Andreeva, Alexandra V¹  Kutuzov, Mikhail A¹ 

[1] Research School of Biological and Molecular Sciences, Oxford Brookes University, Headington, Oxford OX3 OBP, UK

关键词: Protein Ser/Thr phosphatase;    PP7;    Okadaic acid;    Calyculin A;    Fumonisin B1;    Arabidopsis thaliana;   

DOI  :  10.1016/S0014-5793(98)01428-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have recently identified an Arabidopsis thaliana cDNA encoding a putative protein Ser/Thr phosphatase PP7, not closely related to any protein phosphatases in animals or fungi. Here, we describe the characterization of PP7 expressed in a bacterial system. The recombinant protein was inactive unless the longest insert in its catalytic domain was cleaved, suggesting that this insert is an autoinhibitory region. PP7 was resistant to okadaic acid, calyculin and fumonisin B₁, and was stimulated by Mn²⁺ or Fe²⁺, while Ni²⁺ and Zn²⁺ were inhibitory. Polylysine stimulated PP7 activity towards p-nitrophenylphosphate but inhibited activity towards the most efficient protein substrate, myelin basic protein. A tentative model of the control of PP7 activity is proposed.

【 授权许可】

Unknown