Stromal and thylakoid‐bound ascorbate peroxidases are produced by alternative splicing in pumpkin

FEBS Letters

Hayashi, Makoto¹  Nishimura, Mikio¹  Yamaguchi, Katsushi¹  Mano, Shoji¹ 

[1] Department of Cell Biology, National Institute for Basic Biology, Okazaki 444, Japan

关键词: Alternative splicing;    Chloroplast;    Pumpkin (Cucurbita sp. cv. Kurokawa Amakuri Nankin);    Stromal ascorbate peroxidase (EC 1.11.1.11);    Thylakoid-bound ascorbate peroxidase;    APX;    ascorbate peroxidase;    sAPX;    stromal APX;    tAPX;    thylakoid-bound APX;    mbAPX;    microbody APX;    cAPX;    cytosolic APX;    HPR;    hydroxypyruvate reductase;    RT-PCR;    reverse transcription-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(97)00862-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding stromal ascorbate peroxidase (sAPX) was isolated using poly(A)⁺ RNA of pumpkin cotyledons by RT-PCR. The cDNA encodes a polypeptide with 372 amino acids and shares complete sequence identity with pumpkin thylakoid-bound ascorbate peroxidase (tAPX), except for the deletion of a putative membrane spanning region located in the carboxy domain of tAPX. Southern blot hybridization and analysis of intron structure indicated that mRNAs for sAPX and tAPX, whose suborganellar localizations in chloroplasts are different, are produced by alternative splicing. Immunoblot analysis showed that the accumulation of sAPX and tAPX was differently regulated during germination and subsequent greening of pumpkin cotyledons.

【 授权许可】

Unknown