Direct action of nitric oxide on osteoblastic differentiation

FEBS Letters

Oida, Shinichiro¹  Koizumi, Toshiyuki³  Takato, Tuyoshi³  Toyo-oka, Teruhiko²  Shin, Wee Soo²  Hikiji, Hisako³ 

[1] Department of Oral Biochemistry, Faculty of Dentistry, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113, Japan;The Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113, Japan;Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of Tokyo, Tokyo 113, Japan

关键词: NO;    NOS gene expression;    Osteoblastic differentiation;    TNF-α;    IL-1β;    AIPase;    alkaline phosphatase;    cGMP;    cyclic guanosine monophosphate;    diethylamine NONOate;    Ethanamine;    N-ethyl-;    compound with 1;    1-diethyl-2-hydroxy-2-nitrosohydrazine;    EIA;    enzyme immunoassay;    IL-1β;    Interleukin-1β;    l-NMMA;    NG-monomethyl-l-arginine;    NO;    nitric oxide;    NOS;    nitric oxide synthase;    PGE2;    prostaglandin E2;    RT-PCR;    reverse transcription-polymerase chain reaction;    SNP;    sodium nitroprusside;    TNF-α;    tumor necrosis factor-α;   

DOI  :  10.1016/S0014-5793(97)00597-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of nitric oxide (NO) on osteoblastic differentiation was examined in cultured mouse osteoblasts. Interleukin-1β and tumor necrosis factor-α expressed inducible NO synthase gene with little effect on constitutive NO synthase gene. These cytokines increased NO production, which was inhibited by l-NMMA pretreatment, and decreased alkaline phosphatase (AIPase) activity, which was not restored by l-NMMA. Furthermore, NO donors, sodium nitroprusside and NONOate dose-dependently elevated AIPase activity and expression of osteocalcin gene. These results suggest that NO directly facilitates osteoblastic differentiation and the cytokine-induced inhibition of AIPase activity is mediated via mechanism other than NO.

【 授权许可】

Unknown