Activation of NF‐κB in human osteoblasts by stimulators of bone resorption

FEBS Letters

Ali, Nadire N¹  Winyard, Paul G¹  Gilston, Vanessa¹ 

[1] Bone and Joint Research Unit, St. Bartholomew's and the Royal London School of Medicine and Dentistry, 25–29 Ashfield Street, London E1 2AD, UK

关键词: Osteoblast;    Resorptive signalling;    Parathyroid hormone;    Interleukin-1;    Tumour necrosis factor;    Nuclear factor κB;    NF-κB;    nuclear factor κB;    TRANCE;    TNF-related activation-induced cytokine;    RANK;    receptor activator of NF-κB;    ODF;    osteoclast differentiation factor;    OPG;    osteoprotegerin;    OCIF;    osteoclastogenesis inhibitory factor;    IL-;    interleukin-;    TNF-α;    tumour necrosis factor-α;    PTH;    parathyroid hormone;    PGE2;    prostaglandin E2;    COX-2;    cyclooxygenase-2;    NO;    nitric oxide;    iNOS;    inducible nitric oxide synthase;    1;    25-(OH)2 vitamin D3;    1;    25-dihydroxyvitamin D3;    TCM;    tissue culture medium;    FCS;    foetal calf serum;    NE;    nuclear extract;    EMSA;    electrophoretic mobility shift assay;   

DOI  :  10.1016/S0014-5793(99)01370-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Several bone resorptive stimuli affect osteoclasts indirectly by modulating the production and release of osteoblastic factors. Using electrophoretic mobility shift assays, we found that not only tumour necrosis factor-α (TNF-α) but also interleukin-1β and parathyroid hormone (PTH) caused dose and time-related increases in nuclear factor κB (NF-κB)-DNA binding in Saos-2 human osteoblastic (hOB) cells. Activation of NF-κB by TNF-α was reproduced in primary hOBs. In contrast, consistent with their previously reported lack of response to steroid hormones, Saos-2 cells did not respond to 1,25-dihydroxyvitamin D₃. We suggest that NF-κB activation in osteoblastic cells constitutes an important pathway in osteoblast-mediated resorptive signalling.

【 授权许可】

Unknown