Interleukin 1‐ and tumor necrosis factor‐stimulation of prostaglandin E2 synthesis in MDCK cells, and potentiation of this effect by cycloheximide

FEBS Letters

Leighton, Jonathan D.¹  Pfeilschifter, Josef¹ 

[1] Research Department, Pharmaceuticals Division, Ciba-Geigy AG, Basel, Switzerland

关键词: Interleukin-1;    TNFα;    Prostaglandin-E2;    Madin-Darby canine kidney cell;    Cycloheximide;    IL-1;    interleukin-1;    TNF;    tumor necrosis factor;    PGE2;    prostaglandin E2;   

DOI  :  10.1016/0014-5793(90)80029-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of interleukin (IL)- lα, IL- 1β and TNFα on prostaglandin-E2 synthesis in Madin-Darby canine kidney (MDCK) cells were investigated. IL-lβ time- and dose-dependently stimulated prostaglandin-E2 synthesis. While TNFα produced a comparatively small but significant stimulation of PGE2 release, coincubation of IL-lβ with TNFα produced a marked synergistic stimulation of PGE2 release. The effect of IL-lβ and of IL-1β and TNFα was apparent as early as after 2 h of incubation. The enhanced PGE2 synthesis was inhibited by indomethacin as well as actinomycin D, while cycloheximide surprisingly potentiated PGE2 synthesis in response to both IL-1β and TNFα. IL-1α alone was ineffective in stimulating a significant release of PGE2 at concentrations as high as 10 nM. However, it also showed a marked synergistic interaction with TNFα in stimulating PGE2 release.

【 授权许可】

Unknown