Ganglioside GM2 is substrate for a sialidase in MDCK cells

FEBS Letters

Marchesini, Sergio¹  Piccinotti, Alberto¹  Preti, Augusto¹  Benaglia, Giuliana¹  Bresciani, Roberto¹ 

[1] Department of Biomedical Sciences and Biotechnology, University of Brescia, Via Valsabbina 19, 25123 Brescia, Italy

关键词: Madin-Darby canine kidney cell;    Asialo-monosialoganglioside 2;    Sialidase;    Fluorescent ganglioside;    N-Lissamine rhodamine;    Ganglioside nomenclature follows the suggestions of Svennerholm (Adv. Exp. Med. Biol. (1980) 125;    11–12) and the IUPAC-IUB recommendations (Lipids (1977) 12;    455–468);    Cer;    ceramide;    Glc-Cer;    glucosyl-ceramide;    Lac-Cer;    lactosyl-ceramide;    LRh;    lissamine rhodamine;   

DOI  :  10.1016/S0014-5793(98)00510-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

GM1 ganglioside carrying a fluorescent fatty acid in substitution of the natural one, has been administered to cultured Madin-Darby canine kidney (MDCK) cells for different pulse times (0.5–24 h), and its metabolic fate was followed. The fluorescent GM2, asialo-GM2, asialo-GM1 and ceramide were the only detectable metabolites. The complete absence of fluorescent GM3 is consistent with the presence in these cells of a sialidase working on GM1 and GM2 gangliosides. After treatment of the cells with chloroquine the fluorescent GM1 remained essentially undegraded, indicating a catabolic processing at lysosomal level.

【 授权许可】

Unknown