Induction of apoptotic cell death by pancreatitis‐associated ascitic fluid in Madin‐Darby canine kidney cells

FEBS Letters

Nishikawa, Junsuke¹  Hori, Yuichi¹  Takeyama, Yoshifumi¹  Ueda, Takashi¹  Ueno, Nozomi¹  Yamamoto, Masahiro¹  Saitoh, Yoichi¹ 

[1]First Department of Surgery, Kobe University School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650, Japan

关键词: Acute pancreatitis;    Ascitic fluid;    Apoptosis;    Multiple organ failure;    Madin-Darby canine kidney cell;   

DOI  :  10.1016/0014-5793(95)00999-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the cytotoxicity on Madin-Darby canine kidney (MDCK) cells of pancreatitis-associated ascitic fluid (PAAF) collected from rats with experimental necrotizing pancreatitis. PAAF reduced viability of MDCK cells in a time- and dose-dependent manner. We detected DNA fragmentation on the PAAF-treated MDCK cells, indicating that the cytocidal action of PAAF is via apoptosis. From the results obtained, we conclude that PAAF contains factor(s) inducing apoptosis on MDCK cells, and we assume that apoptotic cell death is involved in the mechanism of organ failure in acute pancreatitis.

【 授权许可】

Unknown