Interaction of the G‐protein G11α with receptors and phosphoinositidase C:

FEBS Letters

Parenti, Marco²  Wise, Alan¹  Milligan, Graeme¹ 

[1] Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK;Dipartimento di Pharmacologia, Università di Milano, Via Vanvitelli 32, 20129 Milan, Italy

关键词: G protein;    Acylation;    Receptor;    Phospholipase C;    Inositol;   

DOI  :  10.1016/S0014-5793(97)00300-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Wild-type and palmitoylation defective mutants of the murine G protein G₁₁α were transfected into HEK293 cells. Wild-type G₁₁α was membrane associated, Cys9Ser Cys10Ser G₁₁α was present in the soluble fraction whilst both Cys9Ser G₁₁α and Cys10Ser G₁₁α were distributed between the fractions. Expression of the rat TRH receptor resulted in agonist stimulation of inositol phosphate accumulation. The degree of stimulation produced by TRH following co-transfection of the palmitoylation-resistant forms of G₁₁α compared to the wild-type protein correlated with the amount of membrane-associated G protein.

【 授权许可】

Unknown