Two α subunits of the Gq class of G proteins stimulate phosphoinositide phospholipase C‐β1 activity

FEBS Letters

Exton, John H.¹  Taylor, Stephen J.² 

[1] Howard Hughes Medical Institute, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA;Cornell University, Section of Biochemistry, Ithaca, NY 14853, USA

关键词: G-protein;    Phosphoinositide;    Phospholipase C;    Gq;    G protein;    guanine nucleotide binding regulatory protein;    PLC;    phosphoinositide-specific phospholipase C;    GPA;    G protein phospholipase C activator;    PTX;    pertussis toxin;    GTPγS;    guanosine 5′-o-[3-thiotriphosphate];    HEPES;    4-[2-hydroxyethyl]-1-piperazineethane sulphonic acid;   

DOI  :  10.1016/0014-5793(91)80976-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two G protein α subunits were detected in preparations of GTPγS-dependent, phosphoinositide-specific phospholipase C-activating proteins from bovine liver membranes. Partial resolution of the two α subunits, of molecular mass 42 and 43 kDa, was achieved by Mono Q chromatography. Quantitation of the levels of each α subunit and reconstitution assays demonstrated that each possessed stimulatory activity towards the β1 isozyme of phospholipase C. Immunoblot analysis showed that the 42 kDa protein was immunologically related to α_q, whereas the 43 kDa protein was related to α₁₁, another member of the G_q class. The data thus show that two different α subunits of the G_q class of G proteins stimulate phospholipase C-β1 Activity.

【 授权许可】

Unknown