Two species of phospholipase C isolated from lymphocytes produce specific ratios of inositol phosphate products

FEBS Letters

Carter, Helen R.¹  Bird, Ian M.¹  Smith, Anthony D.¹ 

[1] Courtauld Institute of Biochemistry, Middlesex Hospital Medical School. Mortimer Street, London W1P 7PN, England

关键词: Phospholipase C;    Phosphoinositide;    Inositol phosphate;    Lymphocyte tranformation;    PtdIns;    phosphatidylinositol;    PtdIns 4P;    phosphatidylinositol 4-phosphate;    PtdIns 4;    5P2;    phosphatidylinositol 4;    5-bisphosphate;    GroPIns;    glycerophosphoinositol;    Ins 1P;    inositol 1-phosphate;    Ins 2P;    inositol 2-phosphate;    Ins 1;    4;    5P3;    inositol 1;    4;    5-tris-phosphate;    Ins 1;    2>P;    inositol 1;    2-cyclic phosphate;    Ins (1;    2>P)4;    5P3;    inositol 1;    2-cyclic 4;    5-trisphosphate;    PMSF;    phenylmethylsulphonyl fluoride;    DTE;    dithioerythritol;   

DOI  :  10.1016/0014-5793(86)81380-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chromatography of the soluble porcine lymphocyte phospholipase C on cellulose phosphate resolves the activity into two species. An HPLC method is described for separating the enzyme products. Ins 1,2 > P and Ins 1P. Use of these methods reveals that the two iso-enzymes liberate, with a high degree of reproducibility, characteristic ratios of the two products. This suggests that the amount of each product produced is an inherent property of the enzyme mechanism.

【 授权许可】

Unknown