Phosphoinositides in membranes that build up the triads of rabbit skeletal muscle

FEBS Letters

Heilmeyer, Ludwig M.G.¹  Milting, Hendrik¹  Thieleczek, Rolf¹ 

[1] Institut für Physiologische Chemie, Abteilung für Biochemie Supramolekularer Systeme, Ruhr-Universität, D-44780 Bochum, Germany

关键词: Phosphoinositide;    PtdIns(4;    5)P2;    Phospholipase C;    Inositol 1;    4;    5-trisphosphate;    Triad;    Skeletal muscle;    GroPIns;    glycerophosphoinositol;    GroPIns4P;    glycerophosphoinositol 4-phosphate;    GroPIns(4;    5)P2;    glycerophosphoinositol 4;    5-bisphosphate;    HPLC;    high-performance liquid chromatography;    HPTLC;    high-performance thin-layer chromatography;    4-methylum-belliferyl phosphate;    MUM;    Ins(1;    4;    5)P3;    inositol 1;    4;    5-trisphosphate;    PtdIns;    phosphatidylinositol;    PtdIns4P;    phosphatidylinositol 4-phosphate;    PtdIns(4;    5)P2;    phosphatidylinositol 4;    5-bisphosphate;    PLC;    PtdIns(4;    5)P2-directed phospholipase C;    SR;    sarcoplasmic reticulum;    TC;    terminal cisternae;    TT;    transverse tubules;   

DOI  :  10.1016/0014-5793(94)00440-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The total membrane concentrations of PtdIns, PtdIns4P, and PtdIns(4,5)P₂ contribute to the functional capacity of the Ins(1,4,5)P₃ signalling system which is operating in skeletal muscle but the function of which is still unknown. Total amounts of these phosphoinositides have been determined in purified membranes of transverse tubules (TT) and terminal cisternae (TC) of the sarcoplasmic reticulum (SR) of rabbit skeletal muscle. PtdIns and PtdIns4P have been detected in both membrane systems whereas PtdIns(4,5)P₂ (290 μmol/mol phospholipid) is confined only to TT. A much greater pool of PtdIns(4,5)P₂ seems, however, to be located in the sarcolemma away from the triadic junction.

【 授权许可】

Unknown