Kidney produces a novel acylated peptide, ghrelin

FEBS Letters

Hosoda, Kiminori¹  Ariyasu, Hiroyuki¹  Mukoyama, Masashi¹  Yahata, Kensei¹  Kangawa, Kenji²  Yoshimoto, Akihiro¹  Kojima, Masayasu²  Nakao, Kazuwa¹  Sugawara, Akira¹  Hosoda, Hiroshi²  Mori, Kiyoshi¹  Takaya, Kazuhiko¹ 

[1] Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan;Department of Biochemistry, National Cardiovascular Center Research Institute, Osaka 565-8565, Japan

关键词: Ghrelin;    Acylation;    Growth hormone;    Growth hormone secretagogue;    Receptor;    Kidney;    GH;    growth hormone;    IGF;    insulin-like growth factor;    GHS;    growth hormone secretagogue;    TFA;    trifluoroacetic acid;    RIA;    radioimmunoassay;    RP-HPLC;    reverse-phase high performance liquid chromatography;    RT-PCR;    reverse transcription-PCR;    C-IR;    carboxyl-terminal ghrelin immunoreactivity;    N-IR;    amino-terminal ghrelin immunoreactivity;   

DOI  :  10.1016/S0014-5793(00)02308-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ghrelin is a novel growth hormone-releasing peptide with a unique acylated structure. Here we reveal that prepro-ghrelin gene is expressed in the mouse kidney and glomerulus. We also show by reverse-phase high performance liquid chromatography coupled with radioimmunoassay that the mouse kidney does produce ghrelin. The ghrelin immunoreactivity in the mouse kidney is 6.79±0.48 fmol/mg (n=5), which is much more abundant than that in the mouse plasma of 0.339±0.029 fmol/μl (n=6). Furthermore, prepro-ghrelin gene is expressed in cultured rat mesangial cells, fibroblast-like NRK-49F cells and mouse podocytes, but not in rat epithelial cell-like NRK-52E cells. Ghrelin receptor gene is also expressed in the rat kidney. These findings demonstrate that the kidney, glomerulus and renal cells express prepro-ghrelin gene and ghrelin is produced locally in the kidney, and suggest the endocrine and/or paracrine roles of ghrelin in the kidney.

【 授权许可】

Unknown