Laccase is essential for lignin degradation by the white‐rot fungus Pycnoporus cinnabarinus

FEBS Letters

Eriksson, Karl-Erik L.²  Eggert, Claudia¹  Temp, Ulrike¹ 

[1]Institute of General Microbiology and Microbe Genetics, Friedrich-Schiller University Jena, Neugasse 24, 07743 Jena, Germany

[2]Department of Biochemistry and Molecular Biology, Center for Biological Resource Recovery, University of Georgia, A214 Life Sciences Building, Athens, GA 30602-7229, USA

关键词: Lignin degradation;    White-rot;    Laccase;    Laccase-less mutant;    Pycnoporus cinnabarinus;    ABTS;    2;    2′-azino-bis-(3-ethylthiazoline-6-sulfonate);    DHP;    dehydrogenative polymer;    DMF;    dimethylformamide;    3-HAA;    3-hydroxyanthranilate;    LiP;    lignin peroxidase;    MnP;    manganese-dependent peroxidase;    Lac− strain;    laccase-less mutant;    Pc wt;    Pycnoporus cinnabarinus wild-type;   

DOI  :  10.1016/S0014-5793(97)00301-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The white-rot fungus, Pycnoporus cinnabarinus, provides an excellent model organism to elucidate the controversial role of laccase in lignin degradation. P. cinnabarinus produces laccase in one isoform as the predominant phenoloxidase in ligninolytic cultures, and neither LiP nor MnP are secreted. Yet, P. cinnabarinus degrades lignin very efficiently. In the present work, we show that laccase-less mutants of P. cinnabarinus were greatly reduced in their ability to metabolize ¹⁴C ring-labeled DHP. However, ¹⁴CO₂ evolution in these mutant cultures could be restored to levels comparable to those of the wild-type cultures by addition of purified P. cinnabarinus laccase. This clearly indicates that laccase is absolutely essential for lignin degradation by P. cinnabarinus.

【 授权许可】

Unknown