FEBS Letters | |
Participation of Mn(II) in the catalysis of laccase, manganese peroxidase and lignin peroxidase from Phlebia radiata | |
Hatakka, Annele1  Lundell, Taina1  | |
[1] Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, PO Box 27, FIN-00014 Viikki, Finland | |
关键词: Lignin degradation; White-rot fungus; Enzymatic oxidation; Peroxidase; Laccase; Manganese(II); LiP; lignin peroxidase; MnP; manganese peroxidase; ABTS; 2; 2′-azino-di-(3-ethylbenz-thiazoline-6-sulfonate); | |
DOI : 10.1016/0014-5793(94)00627-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Oxidation capacities of laccase, manganese peroxidase (MnP) and lignin peroxidase (LiP) from Phlebia radiata were compared using non-phenolic (veratryl alcohol and ABTS) and phenolic (syringaldazine, vanillalacetone and Phenol red) compounds as reducing substrates. The effect of Mn(II) on enzyme reactions was also studied. Highest specific activities were recorded with laccase in the oxidation of phenolic compounds or ABTS and irrespective of MN(II) concentration. LiP and MnP oxidized all these substrates but only the catalysis of MnP was dependent upon Mn(II). Only LiP clearly oxidized veratryl alcohol. However, Mn(II) interfered with this reaction by repressing veratraldehyde formation. These results point to multiple participation of manganese ions, either as a reducing (Mn(II)) or oxidizing (Mn(III)) agent in the enzymatic reactions.
【 授权许可】
Unknown
【 预 览 】
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