A novel combination of prosthetic groups in a fungal laccase; PQQ and two copper atoms

FEBS Letters

Niku-Paavola, Marja-L.¹  Haltia, Tuomas³  Karhunen, Eija³  Duine, Johannis A.²  Viikari, Liisa³  van der Meer, Robert A.² 

[1] VTT, Biotechnical Laboratory, Espoo, Finland;Department of Microbiology and Enzymology, Delft University of Technology, Delft, The Netherlands;Department of Medical Chemistry, University of Helsinki, Finland

关键词: Laccase;    PQQ;    Copper enzyme;    White rot fungus;    Lignin degradation;    PQQ;    pyrroloquinoline quinone;    EPR;    electron paramagnetic resonance;    ABTS;    2;    2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid);    EDTA;    ethylenediaminetetraacetic acid;    AAS;    atomic absorption spectrometry;    GLC-MS;    gas liquid chromatography-mass spectrometry;    SDS;    sodium dodecyl sulphate;    IEF;    isoelectric focusing;   

DOI  :  10.1016/0014-5793(90)80273-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Extracellular laccase (benzenediol:oxygen oxidoreductase EC 1.10.3.2) from the lignin-degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR spectrum exhibits features of type 1 and type 2 copper atoms. In the enzymatic reaction 4 molecules of lignin model compound, coniferyl alcohol, are oxidized per molecule of oxygen reduced to water. During the reaction coniferyl alcohol is transformed to dilignols.

【 授权许可】

Unknown