Lignocellulose-converting enzyme activity profiles correlate with molecular systematics and phylogeny grouping in the incoherent genus Phlebia (Polyporales, Basidiomycota)

BMC Microbiology

Research Article

Jaana Kuuskeri¹  Taina Lundell¹  Miia R. Mäkelä¹  Ilona Oksanen¹  Jarkko Isotalo² 

[1] Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, University of Helsinki, Viikki Biocenter 1, P.O.B. 56, FIN-00014, Helsinki, Finland;Department of Forest Sciences, University of Helsinki, Helsinki, Finland;

关键词: White rot fungus;    Wood decay;    Lignocellulose;    Lignin biodegradation;    Oxidoreductases;    Carbohydrate active enzymes;    Molecular systematics;    Multi-locus phylogeny;    Phlebia;    Polyporales;    Basidiomycota;   

DOI  :  10.1186/s12866-015-0538-x

 received in 2015-01-20, accepted in 2015-09-25,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe fungal genus Phlebia consists of a number of species that are significant in wood decay. Biotechnological potential of a few species for enzyme production and degradation of lignin and pollutants has been previously studied, when most of the species of this genus are unknown. Therefore, we carried out a wider study on biochemistry and systematics of Phlebia species.MethodsIsolates belonging to the genus Phlebia were subjected to four-gene sequence analysis in order to clarify their phylogenetic placement at species level and evolutionary relationships of the genus among phlebioid Polyporales. rRNA-encoding (5.8S, partial LSU) and two protein-encoding gene (gapdh, rpb2) sequences were adopted for the evolutionary analysis, and ITS sequences (ITS1 + 5.8S + ITS2) were aligned for in-depth species-level phylogeny. The 49 fungal isolates were cultivated on semi-solid milled spruce wood medium for 21 days in order to follow their production of extracellular lignocellulose-converting oxidoreductases and carbohydrate active enzymes.ResultsFour-gene phylogenetic analysis confirmed the polyphyletic nature of the genus Phlebia. Ten species-level subgroups were formed, and their lignocellulose-converting enzyme activity profiles coincided with the phylogenetic grouping. The highest enzyme activities for lignin modification (manganese peroxidase activity) were obtained for Phlebia radiata group, which supports our previous studies on the enzymology and gene expression of this species on lignocellulosic substrates.ConclusionsOur study implies that there is a species-level connection of molecular systematics (genotype) to the efficiency in production of both lignocellulose-converting carbohydrate active enzymes and oxidoreductases (enzyme phenotype) on spruce wood. Thus, we may propose a similar phylogrouping approach for prediction of lignocellulose-converting enzyme phenotypes in new fungal species or genetically and biochemically less-studied isolates of the wood-decay Polyporales.

【 授权许可】

CC BY   
© Kuuskeri et al. 2015