`Yellow' laccase of Panus tigrinus oxidizes non‐phenolic substrates without electron‐transfer mediators

FEBS Letters

Myasoedova, Nina¹  Golovleva, Ludmila¹  Leontievsky, Alexey¹  Pozdnyakova, Natalia¹ 

[1] Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), Pushchino, Moscow Region 142292, Russia

关键词: White rot fungus;    Laccase;    Redox-mediator;    Absorption spectra;    Lignin;    ABTS;    2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid);    AAS;    atomic absorption spectroscopy;    EPR;    electron paramagnetic resonance;    MS;    mass spectrometry;    PAGE;    polyacrylamide gel electrophoresis;    TLC;    thin layer chromatography;   

DOI  :  10.1016/S0014-5793(97)00953-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Yellow and blue forms of laccase from solid-state and submerged cultures of Panus tigrinus were isolated. Both laccases had similar molecular masses and specific activity, but yellow laccase had no `blue' maximum in the absorption spectrum. Blue laccase oxidized veratryl alcohol and a non-phenolic dimeric lignin model compound only in the presence of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) as electron-transfer mediator. Yellow laccase catalyzed these reactions without any additional compounds. It is supposed that yellow laccase is formed as a result of blue laccase modification by products of lignin degradation. These compounds might play a role of natural electron-transfer mediators for the oxidation of non-phenolic substances, catalyzed by yellow laccase.

【 授权许可】

Unknown