The interaction between Alzheimer amyloid β(1–40) peptide and ganglioside GM1‐containing membranes

FEBS Letters

Choo-Smith, Lin-P'ing¹  Surewicz, Witold K¹ 

[1] Division of Neuropathology, Department of Pathology, Case Western Reserve University, 2085 Adelbert Road, Cleveland, OH 44106, USA

关键词: β-Amyloid peptide;    Alzheimer's disease;    Ganglioside GM1;    Peptide conformation;    AD;    Alzheimer's disease;    POPC;    1-palmitoyl-2-oleoyl phosphatidylcholine;    POPG;    1-palmitoyl-2-oleoyl phosphatidylglycerol;    POPS;    1-palmitoyl-2-oleoyl phosphatidylserine;    HFIP;    1;    1;    1;    3;    3;    3-hexafluoro-2-propanol;    CD;    circular dichroism;   

DOI  :  10.1016/S0014-5793(96)01504-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction between Alzheimer amyloid peptide Aβ(1–40) and membrane lipids was studied by circular dichroism spectroscopy under the conditions of physiologically relevant ionic strength and neutral pH. The peptide binds to the membranes containing ganglioside G_M1 and upon binding undergoes a conformational transition from random coil to an ordered structure rich in β-sheet. This interaction appears to be ganglioside-specific as no changes in Aβ(1–40) conformation were found in the presence of various phospholipids or sphingomyelin. The isolated oligosaccharide moiety of the ganglioside was ineffective in inducing alterations in the secondary structure of Aβ(1–40). No interaction was observed between ganglioside G_M1 and the N-terminal peptide fragment Aβ(1–28). Binding to the ganglioside is likely to modulate the neurotoxic and/or amyloidogenic properties of Aβ(1–40).

【 授权许可】

Unknown