The structure, function and distribution of the mouse TWIK‐1 K+ channel

FEBS Letters

Lazdunski, Michel¹  Lesage, Florian¹  Reyes, Roberto¹  Duprat, Fabrice¹  Heurteaux, Catherine¹  Fink, Michel¹  Lauritzen, Inger¹ 

[1] Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, 660, route des Lucioles, Sophia Antipolis, 06560 Valbonne, France

关键词: Heterologous expression;    Xenopus oocyte;    In situ hybridization;    Two P domains;   

DOI  :  10.1016/S0014-5793(96)01491-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The two P domain K⁺ channel mTWIK-1 has been cloned from mouse brain. In Xenopus oocytes, mTWIK-1 currents are K⁺-selective, instantaneous, and weakly inward rectifying. These currents are blocked by Ba²⁺ and quinine, decreased by protein kinase C and increased by internal acidification. The apparent molecular weight of mTWIK-1 in brain is 81 kDa. A 40 kDa form is revealed after treatment with a reducing agent, strongly suggesting that native mTWIK-1 subunits dimerize via a disulfide bridge. TWIK-1 mRNA is expressed abundantly in brain and at lower levels in lung, kidney, and skeletal muscle. In situ hybridization shows that mTWIK-1 expression is restricted to a few brain regions, with the highest levels in cerebellar granule cells, brainstem, hippocampus and cerebral cortex.

【 授权许可】

Unknown