The Use of Response Surface Method in Optimization of Levan Production by Heterologous Expressed Levansucrase from Halophilic Bacteria Bacillus licheniformis BK2

3rd International Conference on Biomass: Accelerating the Technical Development and Commercialization for Sustainable Bio-based Products and Energy

生物科学;能源学;经济学

Permatasari, N.U.^1,2 ; Ratnaningsih, E.^1 ; Hertadi, R.^1

Biochemistry Research Division, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Indonesia^1

Chemistry Departement, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Indonesia^2

关键词: Bacillus licheniformis;    Catalytic reactions;    Central composite designs;    FTIR and NMR spectroscopy;    Halophilic bacteria;    Heterologous expression;    Response surface method;    Sucrose concentration;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/209/1/012015/pdf
DOI  :  10.1088/1755-1315/209/1/012015

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

Response surface method (RSM) is a recent common method used to identify culture condition for optimal production of particular metabolite. In the present study, RSM is used to optimize a catalytic reaction in levan production by heterologous expression of lsbl-bk2 gene isolated from halophilic bacteria Bacillus licheniformis. Levan is a polyfructose polymer produced from sucrose by the action of extracellular levansucrase secreted by the microorganism. Three factors for levan production, namely sucrose concentration, pH, and temperature of reaction were optimized by full factorial and central composite designs in RSM. The result indicated that the optimum in vitro condition for levan production was achieved when the levansucrase catalytic reaction was performed at 32?C, pH 8, in 12% (w/v) sucrose solution. Levan produced by this procedure was verified by FTIR and NMR spectroscopies.