Functional expression of a plant plasma membrane transporter in Xenopus oocytes

FEBS Letters

Leigh, R.A.¹  Miller, A.J.¹  Boorer, K.J.¹  Forde, B.G.¹ 

[1] Department of Biochemistry and Physiology, AFRC-IACR, Rothamsted Experimental Station, Harpenden, Herts. AL5 2JQ, UK

关键词: Arabidopsis thaliana;    Hexose transporter;    Proton symporter;    Heterologous expression;    Xenopus oocyte;    3-OMG;    3-O-methylglucose;    MBS;    modified Barth's saline;    SDS;    sodium dodecyl sulphate;   

DOI  :  10.1016/0014-5793(92)80431-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A full-length cDNA clone for the H⁺/hexose co-transporter (STP1) from Arabidopsis thaliana has been transcribed in vitro and the mRNA injected into Xenopus oocytes. Under optimized conditions, oocytes injected with the STPI mRNA accumulated 3-O-[methyl-¹⁴C]glucose at rates of more than a 1000-fold greater than water-injected control oocytes. A hexose-elicited depolarization of the oocyte membrane potential was demonstrated, and uptake was shown to be stimulated by low external pH, confirming the activity of a H⁺/hexose co-transport system. This is the first example of the functional expression of a plant membrane transporter in oocytes.

【 授权许可】

Unknown