FEBS Letters | |
Functional expression of a plant plasma membrane transporter in Xenopus oocytes | |
Leigh, R.A.1  Miller, A.J.1  Boorer, K.J.1  Forde, B.G.1  | |
[1] Department of Biochemistry and Physiology, AFRC-IACR, Rothamsted Experimental Station, Harpenden, Herts. AL5 2JQ, UK | |
关键词: Arabidopsis thaliana; Hexose transporter; Proton symporter; Heterologous expression; Xenopus oocyte; 3-OMG; 3-O-methylglucose; MBS; modified Barth's saline; SDS; sodium dodecyl sulphate; | |
DOI : 10.1016/0014-5793(92)80431-F | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A full-length cDNA clone for the H+/hexose co-transporter (STP1) from Arabidopsis thaliana has been transcribed in vitro and the mRNA injected into Xenopus oocytes. Under optimized conditions, oocytes injected with the STPI mRNA accumulated 3-O-[methyl-14C]glucose at rates of more than a 1000-fold greater than water-injected control oocytes. A hexose-elicited depolarization of the oocyte membrane potential was demonstrated, and uptake was shown to be stimulated by low external pH, confirming the activity of a H+/hexose co-transport system. This is the first example of the functional expression of a plant membrane transporter in oocytes.
【 授权许可】
Unknown
【 预 览 】
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RO201912020296300ZK.pdf | 382KB | download |