Acetylation of ribosomal protein S5 affected by defects in the central pseudoknot in 16S ribosomal RNA?

FEBS Letters

Pleij, Cornelis W.A²  Poot, Raymond A²  van Duin, Jan²  Jeeninga, Rienk E¹ 

[1] Department of Biochemistry and Molecular Biology, IMBW, BioCentrum Amsterdam, Vrije Universiteit, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands;Leiden Institute of Chemistry, Department of Biochemistry, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands

关键词: 16S rRNA;    Ribosomal protein;    Acetylation;    Ribosome;    Pseudoknot;   

DOI  :  10.1016/S0014-5793(96)01467-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have analyzed the ribosomal protein profile of Escherichia coli 30S subunits with the mutation C₁₈A in the central pseudoknot of their 16S ribosomal RNA. This mutation was shown to inhibit translational activity in vivo and to affect ribosome stability in vitro. The majority of the mutant 30S particles were present as free subunits in which a reproducible decrease in amount of proteins S1, S2, S18 and S21 was observed. The protein gels also showed the appearance of a satellite band next to S5. This band reacted with anti-S5 antibodies and had a slightly increased positive charge. The simplest interpretation of these findings, also considering published data, is that the satellite band is S5 with a non-acetylated N-terminal alanine. Underacetylation of S5 due to mutations in the 16S rRNA implies that the modification is performed on the ribosome.

【 授权许可】

Unknown