The effect of aminoacyl‐ or peptidyl‐tRNA at the A‐site on the arrangement of deacylated tRNA at the ribosomal P‐site

FEBS Letters

Karpova, G.G.¹  Babkina, G.T.¹  Matasova, N.B.¹  Bausk, E.V.¹  Graifer, D.M.¹ 

[1] Institute of Organic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk 630090, USSR

关键词: Photoreactive tRNA derivative;    Affinity labelling;    Ribosome;    tRNA-binding site;    Ribosomal protein;   

DOI  :  10.1016/0014-5793(84)81330-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Photoreactive derivatives of E. coli tRNA^Phe bearing arylazido groups on guanine residues (azido-tRNA) were used for affinity labelling of E. coli ribosomes in the region of the P-site when the A-site was either free or occupied by aminoacyl- or peptidyl-tRNA. Corresponding complexes of azido-tRNA with ribosomes and poly(U) were obtained both nonenzymatically and with the use of elongation factors. UV-irradiation of the complexes resulted in labelling of ribosomal proteins (preferentially of 30 S subunit). Proteins S9 and S21 were labelled only when the A-site was free; S14 — only when it was occupied; S11, S13, S19 — in both cases; S5, S7, S12, S20 — in some states.

【 授权许可】

Unknown