Determination of tRNA nucleotide residues directly interacting with proteins in the post‐ and pretranslocated ribosomal complexes

FEBS Letters

Tsvetkova, E.A.¹  Abdurashidova, G.G.¹  Budowsky, E.I.¹ 

[1] Zelinsky Institute of Organic Chemistry, USSR Academy of Sciences, Moscow 117913, USSR

关键词: Escherichia coli ribosome;    tRNA-binding site;    tRNA-protein contacts;    UV-induced tRNA-protein cross-links;    Localization of cross-linked tRNA residues;   

DOI  :  10.1016/0014-5793(90)81202-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nucleotide residues of E.coli tRNA interacting directly with proteins in pre- and posttranslocated ribosomal complexes have been identified by analysis of photo-induced tRNA-protein cross-links. A9, G 18, A26 and U59 residues of NAcPhePhe-tRNA, located in the A_b-site (pretranslocated complex) have been cross-linked with proteins S10, L27, S7 and L2 respectively. In deacylated tRNA, located in the P_b-site, residues C17, G44, C56 and U60 have been cross-linked with proteins L2, L5, L27 and S9 respectively. The G44-L5 cross-link disappeared after translocation (NAc-PhePhe-tRNA located in the P_t-site).

【 授权许可】

Unknown