Direct cross‐linking of heptauridilate to E. coli ribosomes by water‐soluble carbodiimide in the complex stabilized by codon‐anticodon interaction at both A‐ and P‐sites

FEBS Letters

Karpova, G.G.¹  Gimautdinova, O.I.¹  Frolova, S.B.¹  Knorre, D.G.¹ 

[1] Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk 630090, USSR

关键词: mRNA analog;    Affinity labeling;    Ribosome;    Soluble carbodiimide;    Ribosomal protein;    mRNA binding area;   

DOI  :  10.1016/0014-5793(85)80910-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Affinity labelling of E. coli ribosomes is performed by treatment with water-soluble carbodiimide of the complex of ribosomes with (pU)₇, tRNA^Phe at the P-site and with Phe-tRNA^Phe (complex I) and without Phe-tRNA^Phe (complex II) at the A-site. The extent of modification is, respectively, 0.06 and 0.026 mol (pU)₇ per mol ribosomes. Protein S3 is found as a single labelled protein in complex I, whereas S7, S8, L25 are modified in complex II. Thus, in the absence of a large spacer group within the complex stabilized by codonanticodon interactions at both A- and P-sites, a highly selective modification occurs.

【 授权许可】

Unknown