A complex of the soluble interleukin‐6 receptor and interleukin‐6 is internalized via the signal transducer gp130

FEBS Letters

Hemmann, Ulrike¹  Dittrich, Elke¹  Korolenko, Tatjana A.¹  Heinrich, Peter C.¹  Graeve, Lutz¹  Weiergräber, Oliver¹ 

[1] Institut fur Biochemie, Rheinisch-Westfälische Technische Hochschule Aachen, Pauwelstr. 30, 52057 Aachen, Germany

关键词: Interleukin-6;    Soluble interleukin-6 receptor;    gp130;    Endocytosis;    Lysosome;    IL-6;    interleukin-6;    sIL-6R;    soluble interleukin-6 receptor;    MDCK;    Madin-Darby canine kidney;   

DOI  :  10.1016/S0014-5793(96)01305-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In human body fluids a soluble form of the interleukin-6 receptor (sIL-6R) has been found which together with interleukin-6 (IL-6) acts agonistically on cells expressing the signal transducer gp130. The means by which the sIL-6R is removed from the circulation is unknown. Here, we show that a complex of ¹²⁵I-labelled recombinant sIL-6R and IL-6 is internalized by MDCK cells stably transfected with gp130 and by human hepatoma cells HepG2 that endogenously express the IL-6R and gp130. We further show that most of the internalized sIL-6R is degraded within lysosomes. Our studies suggest that cells expressing gp130 are capable of endocytosing an IL-6/sIL6R complex, thereby removing both from the circulation.

【 授权许可】

Unknown