Bafilomycin A1 induces apoptosis in PC12 cells independently of intracellular pH

FEBS Letters

Noto, Masahiko¹  Nishikawa, Yasuko¹  Takizawa, Kaori¹  Waritani, Takaki¹  Ohkuma, Shoji¹  Kinoshita, Kuninori¹  Minemoto, Yuzuru¹ 

[1] Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kanazawa University, Takara-machi, Kanazawa, Ishikawa 920, Japan

关键词: Bafilomycin A1;    Apoptosis;    Vacuolar H+-ATPase;    NH4Cl;    PC12 cell;    ATA;    aurintricarboxylic acid;    BCECF-AM;    2′;    7′-bis(carboxyethyl)-5(6)-carboxyfluorescein tetraacetoxymethylester;    CHX;    cycloheximide;    DMSO;    dimethylsulfoxide;    FDA;    fluorescein diacetate;    HEPES;    N-[2-hydroethyl]piperazine-N′-[2-ethanesulfonic acid];    NOG;    neurite outgrowth;    NGF;    nerve growth factor;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(96)01182-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

PC12 cells growth-arrested with bafilomycin A₁ died showing apoptotic chromatin condensation in the nuclei. The bafilomycin A₁-induced chromatin condensation was preceded by neurite outgrowth (NOG), required higher concentrations of bafilomycin A₁ than NOG, and was suppressed by cycloheximide and aurintricarboxylic acid. NH₄Cl (10 mM), another acidotropic pH perturbing agent, neither induced apoptotic chromatin condensation by itself nor suppressed that induced by bafilomycin A₁, suggesting that bafilomycin A₁-induced apoptosis occurs independently of intracellular pH in PC12 cells.

【 授权许可】

Unknown