The effect ofuncouplers (F)CCCP and NH4Cl on the kinetics of the flash‐induced P515 electrochromic bandshift in spinach chloroplasts

FEBS Letters

Peters, R.L.A.¹  Vredenburg, W.J.¹  van Kooten, O.¹ 

[1] Laboratory of Plant Physiological Research, Argricultural University, Gen. Foulkesweg 72, 6703 BW Wageningen, The Netherlands

关键词: P515;    Electrochromic bandshift;    Uncoupler;    FCCP;    CCCP;    NH4Cl;   

DOI  :  10.1016/0014-5793(84)80971-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of uncouplers of photophosphorylation on the kinetics of the flash-induced P515 electrochromic bandshift was investigated in dark-adapted chloroplasts. It was found that the presence of low concentrations of carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) and carbonyl cyanide m-chloro-phenylhydrazone (CCCP) resulted in the selective suppression of the slow P515 absorbance change (reaction 2), whereas the fast change (reaction 1) was not influenced. In contrast, high concentrations of NH₄Cl did not alter the P515 response with respect to reactions 1 and 2. These results indicate that reaction 2 is specifically sensitive towards uncouplers which exert their function as a proton carrier in the lipophilic phase of the membrane.

【 授权许可】

Unknown