Effect of 6‐ketocholestanol on FCCP‐ and DNP‐induced uncoupling in plant mitochondria

FEBS Letters

Vianello, A.¹  Macrì, F.¹  Braidot, E.¹  Mokhova, E.N.¹ 

[1] Dipartimento di Biologia ed Economia Agro-industriale, Sezione di Biologia Vegetale, Università di Udine; via Cotonificio, 108, I-33100 Udine, Italia

关键词: Uncoupler;    FCCP;    DNP;    6-ketocholestanol;    Steroid hormone;    Plant mitochondria;    δi;    transmembrane electrical potential difference;    BSA;    bovine serum albumin;    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;    DNP;    2;    4-p-dinitrophenol;    EGTA;    ethylene glycol-bis(β-aminoethylether)-N;    N;    N′;    N′;    -tetraacetic acid;    HEPES;    N-(2-hydroxyethylpiperazine-N′-[2-ethanesulfon ic acid]) PMS;    phenazine methosulphate;    Tris;    tris(hydroxymethyl)aminomethane;    kCh;    6-ketocholestanol (5α-cholestan-3β-ol-6-one);    TMPD;    N;    N;    N′;    N′-tetramethyl-p-phenylenediamine dihydrochloride;   

DOI  :  10.1016/0014-5793(95)00431-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Effect of 6-ketocholestanol on FCCP-induced and DNP-induced uncoupling in beef liver and pea stem mitochondria was studied, under experimental conditions at which this steroid abolished the effect of low concentrations of FCCP and other most potent uncouplers in rat mitochondria [Starkov et al. (1994) FEBS Lett., 355, 305–308]. It is shown that, in both types of mitochondria, 6-ketocholestanol prevents or reverses the uncoupling induced by low concentrations of FCCP, but not that caused by high concentrations of FCCP or by any concentration of DNP. Progesterone and male sex hormones, showing recoupling capability in animal mitochondria, appear to be ineffective in the plant system. Cholesterol does not recouple in both animal and plant mitochondria. Plant steroids, such as β-sitosterol and stigmasterol, are also without effect.

【 授权许可】

Unknown