Induction of neurite outgrowth of PC12 cells by an inhibitor of vacuolar H+‐ATPase, bafilomycin A1

FEBS Letters

Ohkuma, Shoji¹  Tamura, Hiro-omi¹ 

[1]Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa 920, Japan

关键词: Bafilomycin A1;    Neurite outgrowth;    Nerve growth factor;    Vacuolar H+-ATPase;    c-fos;    PC12 cell;    NGF;    nerve growth factor;    Bt2cAMP;    dibutyryl adenosine 3′:5′-cyclic monophosphate;    MAP;    microtubule-associated protein;   

DOI  :  10.1016/0014-5793(91)81341-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bafilomycin A₁, a selective inhibitor of vacuolar H⁺-ATPase, induced neurite outgrowth of PC12 cells dose- and time-dependently: more than 50% of the cells extended neurite-like spikes after 24 h treatment with 100 nM bafilomycin A₁. Its dose-response ran roughly parallel to that of a bafilomycin A₁-induced lysosomal pH increase. It was inhibited by LiCl, an inhibitor of the phosphorylation of microtubule-associated proteins and, like nerve growth factor (NGF)-induced neurite outgrowth, it was also inhibited by cycloheximide and actinomycin D. But, unlike the NGF-effect, it was not associated with rapid induction of c-fos.

【 授权许可】

Unknown