Overexpression of Gsα in NG108‐15, neuroblastoma X glioma cells: effects on receptor regulation of the stimulatory adenylyl cyclase cascade

FEBS Letters

Mullaney, Ian¹  Milligan, Graeme¹  Craig Carr, I.¹ 

[1] Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Davidson Building, University of Glasgow, Glasgow G12 8QQ, UK

关键词: Guanine nucleotide binding protein;    Prostanoid;    Adenylyl cyclase;    Neuroblastoma;    G protein;    guanine nucleotide binding protein;    HA;    haernagglutinin;    DMEM;    Dulbecco's modified Eagle's medium;    NECA;    5′-N-ethylcarboxamidoadenosine;   

DOI  :  10.1016/S0014-5793(96)01208-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neuroblastoma X glioma hybrid, NG108-15 cells were stably transfected with an epitope tagged variant of G_sα(HAG_sα). The introduced HA-G_sα was able to interact with the IP prostanoid receptor and was able to stimulate adenylyl cyclase activity as measured by an enhanced capacity of membrane extracts to reconstitute NaF-dependent adenylyl cyclase activity to membranes of S49 lymphoma cyc⁻ cells. Despite this, neither the maximal stimulation nor the potency of agonist ligands at the IP prostanoid, A₂ adenosine or secretin receptors was altered substantially compared to the parental cells although the basal adenylyl cyclase activity was increased. These data indicate that cellular levels of G_sα do not limit signal transduction capacity in NG108-15 cells, whereas enhanced expression of adenylyl cyclase allows greater maximal cAMP generation following receptor activation (MacEwan, D.J., Kim, G.D. and Milligan, G. (1996) Biochem. J. 318, 1033–1039).

【 授权许可】

Unknown