FEBS Letters | |
Inhibition of ICE‐family cysteine proteases rescues murine lymphocytes from lipoxygenase inhibitor‐induced apoptosis | |
Knox, Kirstine A.2  Calder, Philip C.2  Mirzoeva, Olga K.1  Yaqoob, Parveen2  | |
[1] A.N. Belozerzky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russian Federation;Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX13QU, UK | |
关键词: Apoptosis; Lipoxygenase; Caffeic acid derivative; ICE-family cysteine protease; LOX; lipoxygenase; NDGA; nordihydroguaiaretic acid; ETI; 5; 8; 11-eicosatrienoic acid; BHPP; N-benzyl-N-hydroxy-5-phenylpentanamide; CDC; cinnamyl-3; 4-dihydroxy-α-cyanocinnamide; ROS; reactive oxygen species; EEP; ethanol extract of propolis; ICE; interleukin-1β-converting enzyme; | |
DOI : 10.1016/0014-5793(96)01111-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Two lipophilic derivatives of caffeic acid which inhibit lipoxygenase, caffeic acid phenethyl ester (CAPE) and N,N′-dicyclohexyl-O-(3,4-dihydroxycinnamoyl)-isourea (DCHCU), reduced the proliferative response of murine splenocytes to concanavalin A in vitro. Both CAPE and DCHCU induced apoptosis in murine thymocyte cultures as verified by flow cytometry and by visualisation of DNA with acridine orange staining. CAPE-induced apoptosis was inhibited by z-VAD-fmk, an inhibitor of the interleukin-1β-converting enzyme family of cysteine proteases. We suggest that the lipoxygenase pathway of arachidonic acid metabolism plays a role in regulating lymphocyte responses such as proliferation and apoptosis.
【 授权许可】
Unknown
【 预 览 】
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