Inhibition of ICE‐family cysteine proteases rescues murine lymphocytes from lipoxygenase inhibitor‐induced apoptosis

FEBS Letters

Knox, Kirstine A.²  Calder, Philip C.²  Mirzoeva, Olga K.¹  Yaqoob, Parveen² 

[1] A.N. Belozerzky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russian Federation;Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX13QU, UK

关键词: Apoptosis;    Lipoxygenase;    Caffeic acid derivative;    ICE-family cysteine protease;    LOX;    lipoxygenase;    NDGA;    nordihydroguaiaretic acid;    ETI;    5;    8;    11-eicosatrienoic acid;    BHPP;    N-benzyl-N-hydroxy-5-phenylpentanamide;    CDC;    cinnamyl-3;    4-dihydroxy-α-cyanocinnamide;    ROS;    reactive oxygen species;    EEP;    ethanol extract of propolis;    ICE;    interleukin-1β-converting enzyme;   

DOI  :  10.1016/0014-5793(96)01111-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two lipophilic derivatives of caffeic acid which inhibit lipoxygenase, caffeic acid phenethyl ester (CAPE) and N,N′-dicyclohexyl-O-(3,4-dihydroxycinnamoyl)-isourea (DCHCU), reduced the proliferative response of murine splenocytes to concanavalin A in vitro. Both CAPE and DCHCU induced apoptosis in murine thymocyte cultures as verified by flow cytometry and by visualisation of DNA with acridine orange staining. CAPE-induced apoptosis was inhibited by z-VAD-fmk, an inhibitor of the interleukin-1β-converting enzyme family of cysteine proteases. We suggest that the lipoxygenase pathway of arachidonic acid metabolism plays a role in regulating lymphocyte responses such as proliferation and apoptosis.

【 授权许可】

Unknown