Lipoxygenase inhibitors block CD95 ligand‐mediated apoptosis of human malignant glioma cells

FEBS Letters

Gulbins, Erich²  Weller, Michael¹  Lang, Florian²  Dichgans, Johannes¹  Wagenknecht, Bettina¹ 

[1] Department of Neurology, University of Tübingen, School of Medicine, Hoppe-Seyler-Strasse 3, 72076 Tübingen, Germany;Institute of Physiology, University of Tübingen, School of Medicine, 72076 Tübingen, Germany

关键词: Glioma;    CD95;    Nordihydroguaretic acid;    Lipoxygenase;    Apoptosis;    Arachidonic acid;   

DOI  :  10.1016/S0014-5793(97)00468-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CD95 ligand is a cytotoxic cytokine that induces apoptosis. Here we report that CD95-mediated apoptosis of human malignant glioma cells is associated with arachidonic acid (AA) release. Inhibitors of phospholipase A₂, phospholipase C or diacylglycerol lipase have minor effects on AA release and fail to modulate apoptosis. Formation of two AA metabolites generated during CD95-dependent apoptosis is attenuated by the lipoxygenase inhibitor, nordihydroguaretic acid (NDGA). NDGA also blocks CD95 ligand-induced apoptosis. This effect is independent of antioxidant properties of NDGA. Lipoxygenase may thus play a critical role in CD95 ligand-induced apoptosis of human malignant glioma cells.

【 授权许可】

Unknown