CD95 (Fas/APO‐1) induces an increased phosphatidylserine synthesis that precedes its externalization during programmed cell death

FEBS Letters

Pelassy, Claudette¹  Breittmayer, Jean Philippe¹  Aussel, Claude¹ 

[1] INSERM U343, Hôpital de l'Archet, P.O. Box 79, 06202 Nice Cedex 03, France

关键词: Apoptosis;    Programmed cell death;    Fas;    CD95;    Phosphatidylserine;    Annexin V;    T lymphocyte;    Jurkat;    PtdSer;    phosphatidylserine;    PtdEtn;    phosphatidylethanolamine;    PtdCho;    phosphatidylcholine;    PtdIns;    phosphatidylinositol;    Serine-BEES;    serine base exchange enzyme system;   

DOI  :  10.1016/S0014-5793(98)00748-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CD95 (Fas, APO-1)-induced programmed cell death (apoptosis) in T cell lines is accompanied by a rapid flip-flop of phosphatidylserine (PtdSer). Externalization of this phospholipid has been previously recognized as one of the early detectable events of cells undergoing apoptosis. We show here that CD95 induces a rapid (detectable at time <15 min), strong (2.5-fold) but transitory neosynthesis of PtdSer in the Jurkat cell line that precedes its externalization. PtdSer decarboxylation, a mitochondrial specific process, was strongly inhibited by CD95 suggesting that changes in mitochondrial activity take place in the early events of Fas-induced apoptosis and participate in the increased PtdSer synthesis observed. In cells undergoing apoptosis, newly synthesized PtdSer first exposed at the cell surface was in part shed with CD95-induced plasma membrane vesicles, a process that likely explains the transitory effect observed.

【 授权许可】

Unknown