Multiple inhibitory effects of Alzheimer's peptide Aβ1–40 on lipid biosynthesis in cultured human HepG2 cells

FEBS Letters

Koudinova, Natalia V²  Berezov, Temirbolat T²  Koudinov, Alexei R¹ 

[1] Russian Academy of Medical Sciences, Miklucho-Maklay St., 8, Moscow 117198, Russian Federation;Department of Biochemistry, Russian Peoples' Friendship University School of Medicine, Moscow, Russian Federation

关键词: Amyloid beta protein;    Apolipoprotein;    Alzheimer's disease;    Down's syndrome;    Lipid biosynthesis;    Lipoprotein;    Aβ;    amyloid beta;    AD;    Alzheimer's disease;    CSF;    cerebrospinal fluid;    DS;    Down's syndrome;    FBS;    fetal bovine serum;    HDL;    high density lipoprotein;    HSA;    human serum albumin;    sAβ;    soluble form of Aβ;    TLC;    thin-layer chromatography;   

DOI  :  10.1016/0014-5793(96)01042-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Herein we describe the inhibitory effect of the synthetic peptide Aβ1–40, homologous to the major high-density lipoprotein-associated species of Alzheimer's amyloid β protein (Aβ), on lipid biosynthesis in human hepatic HepG2 cells. This culture synthesizes various lipids from [¹⁴C]acetate as a precursor. Treatment of cells with different concentrations of Aβ1–40 decreased the syntheses of various radiolabeled lipid species. The decrease reached saturation at peptide concentrations equal to 10–100 ng ml⁻¹. The lipids whose synthesis was decreased most were free and esterified cholesterol and phospholipids. This inhibitory effect suggests that Aβ protein may modulate physiological intracellular lipid syntheses. It may also be of special importance in the pathological condition, and contribute to the neurodegeneration, in Alzheimer's disease and related disorders.

【 授权许可】

Unknown