Lipoxygenase activity in heart cells

FEBS Letters

Breitbart, Eyal¹  Grossman, Shlomo¹  Sofer, Yossi¹  Shainberg, Asher¹ 

[1] Department of Life Sciences, Bar-Ilan University, Ramat Gan 52900, Israel

关键词: Lipoxygenase;    12-HETE;    15-HETE;    Cardiac muscle;    Cardiomyocyte;   

DOI  :  10.1016/0014-5793(96)01017-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Arachidonic acid (AA) metabolism via the lipoxygenase (LOX) pathway in rat hearts and in cultured rat cardiomyocytes was investigated using 1-[¹⁴C]AA. LOX activity was detected in the microsomal fraction, in the high speed supernatant prepared from rat hearts and in rat cardiomyocyte supernatant. LOX products from all fractions comigrated in thin layer chromatography as 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE. Enzyme linked immunosorbent assay for 12-HETE showed its formation by the microsomal fraction, the ammonium sulfate (AS) pellet, and by rat cardiomyocyte supernatant, while radioimmunoassay for 15-HETE showed its formation only by the AS pellet. The properties of LOX in each fraction are reported here.

【 授权许可】

Unknown