FEBS Letters | |
High‐affinity binding sites for 12(S)‐hydroxy‐5,8,10,14‐eicosatetraenoic acid (12(S)‐HETE) in carcinoma cells | |
Hammarström, Sven1  Herbertsson, Helena1  | |
[1] Department of Cell Biology, Faculty of Health Sciences, Linköping University, S-581 85 Linköping, Sweden | |
关键词: GpIIb/IIIa; 12-HETE; Integrin; Lewis lung carcinoma; Lipoxygenase; Scatchard plot; diHETE; dihydroxyeicosatetraenoic acid; Gp; glycoprotein; HETE; hydroxyeicosatetraenoic acid; HODE; hydroxyoctadecadienoic acid; LLC; Lewis lung carcinoma; LTB4; leukotriene B4; | |
DOI : 10.1016/0014-5793(92)80069-S | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12(S)-HETE) enhances tumor cell adhesion to endothelial cells [Honn et al. (1988) Proc. Soc. Exp. Biol. Med. 189, 130–135]. The effect is correlated to surface expression of an integrin receptor, GpIIb/IIIa. Here, we describe evidence for high-affinity binding of 12(S)-HETE to Lewis lung carcinoma cells. Scatchard plot analyses indicated a single class of sites with apparent K d and B max values of 0.44 nM and 66,000 sites per cell, respectively. Competition experiments with unlabeled compounds shod d that the binding was reversible and saturable as well as stereo- and regiospecific. The 12(S)-HETE binding, demonstrated here, might be an important step in a series of events controlling surface expression of integrin receptors.
【 授权许可】
Unknown
【 预 览 】
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