High‐affinity binding sites for 12(S)‐hydroxy‐5,8,10,14‐eicosatetraenoic acid (12(S)‐HETE) in carcinoma cells

FEBS Letters

Hammarström, Sven¹  Herbertsson, Helena¹ 

[1] Department of Cell Biology, Faculty of Health Sciences, Linköping University, S-581 85 Linköping, Sweden

关键词: GpIIb/IIIa;    12-HETE;    Integrin;    Lewis lung carcinoma;    Lipoxygenase;    Scatchard plot;    diHETE;    dihydroxyeicosatetraenoic acid;    Gp;    glycoprotein;    HETE;    hydroxyeicosatetraenoic acid;    HODE;    hydroxyoctadecadienoic acid;    LLC;    Lewis lung carcinoma;    LTB4;    leukotriene B4;   

DOI  :  10.1016/0014-5793(92)80069-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12(S)-HETE) enhances tumor cell adhesion to endothelial cells [Honn et al. (1988) Proc. Soc. Exp. Biol. Med. 189, 130–135]. The effect is correlated to surface expression of an integrin receptor, GpIIb/IIIa. Here, we describe evidence for high-affinity binding of 12(S)-HETE to Lewis lung carcinoma cells. Scatchard plot analyses indicated a single class of sites with apparent K _d and B _max values of 0.44 nM and 66,000 sites per cell, respectively. Competition experiments with unlabeled compounds shod d that the binding was reversible and saturable as well as stereo- and regiospecific. The 12(S)-HETE binding, demonstrated here, might be an important step in a series of events controlling surface expression of integrin receptors.

【 授权许可】

Unknown