Stereoselective carbonyl reductases from rat skin and leukocyte microsomes converting 12‐ketoeicosatetraenoic acid to 12(S)‐HETE

FEBS Letters

Falgueyret, Jean-Pierre¹  Riendeau, Denis¹  Leblanc, Yves¹ 

[1] Merck Frosst Centre for Therapeutic Research, PO Box 1005, Pointe Claire-Dorval, Québec, H9R 4P8 Canada

关键词: Carbonyl reductase;    12-Ketoeicosatetraenoic acid;    12-Hydroxyeicosatetraenoic acid;    Leukocyte;    Skin;    12-HETE;    12-hydroxy-5;    8;    10;    14-eicosatetraenoic acid;    12-HPETE;    12-hydroperoxy-5;    8;    10;    14-eicosatetraenoic acid;    12-KETE;    12-keto-5(Z);    8(Z);    10(E);    14(Z)-eicosatetraenoic acid;   

DOI  :  10.1016/0014-5793(90)80188-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cell-free preparations from rat polymorphonuclear leukocytes and skin were found to catalyze the reduction of 12-keto-5,8,10,14-eicosatetraenoic acid (12-KETE) to 12-hydroxyeicosatetraenoic acid (12-HETE). The reductase activity was associated with the microsomal fraction and showed a marked preference for NADH over NADPH as reducing cofactor. Characterization of the reaction product by chiral phase HPLC of the methyl ester derivative indicated that 12-KETE reduction generated almost exclusively 12(S)-HETE. The results demonstrate that rat skin and leukocyte microsomes possess an NADH-dependent 12-KETE reductase activity that forms 12(S)-HETE as a major product. The identification of Stereoselective 12-KETE reductases provides a basis for further defining the role these enzymes may play in the regulation of 12-KETE levels and in the protection against degradation of 12-KETE to the pro-inflammatory 12(R)-HETE by selectively generating 12-HETE of the S configuration.

【 授权许可】

Unknown