The occurance of carbonyl reduction in continuous cell lines emphasizes the essentiality of this metabolic pathway

FEBS Letters

Gebel, Thomas¹  Maser, Edmund¹  Netter, Karl J.¹ 

[1] Department of Pharmacology and Toxicology, School of Medicine, University of Marburg, Lahnberge, D-3550 Marburg/Lahn, Germany

关键词: Metyrapone (MPON);    Carbonyl reductase;    Reductive metabolism;    Cell culture;   

DOI  :  10.1016/0014-5793(91)80513-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using the ketone compound metyrapone (MPON) as a substrate for carbonyl reduction it has been verified for the first time that various permanent cell lines in culture express carbonyl reducing activity. This is even true for the dedifferentiated and fibroblasloid cell line V79, emphasizing the essentiality of this metabolic pathway. MPON reducing enzyme activities are located in the endoplasmic reticulum as well as in the cytoplasm of the cells. Compared to MPON-reductase in rat liver microsomes, no immunological homology to microsomal C2REV7 rat liver hepatoma cell MPON-reductase could be detected, indicating differences in antigenic determinants between the enzymes of the solid organ and respective cells in continuous culture.

【 授权许可】

Unknown